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The fresh tissue was thoroughly homogenised in liquid nitrogen following extraction in ice-cold buffer containing100 mM TrisCl (pH 7.8) buffer containing 10 mM MgCl 2 , 1 mM PMSF, 10 mM DTT, 100 mM EDTA, and 2% PVP, 0.1% protease inhibitor
(2024) 38:e23421
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502 As for the risk of recurrence among women who attempted to conceive, a recent prospective trial indicated that among HR+ early breast cancer patients, temporary interruption of endocrine therapy to attempt pregnancy would not induce a higher rate of breast cancer recurrence (8.9%) compared to the control group (9.2%)
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