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After a steepest descent minimization (5,000 steps), the systems were equilibrated in several steps ((1) 500-ps simulation with constant number of particles, volume and temperature, and with a time step of t = 0.5 fs

However, treatment with curcumin in PCOS rats significantly reduced ROS ( p = 0.005) and malondialdehyde ( p = 0.004) levels while increasing superoxide dismutase ( p = 0.04), glutathione peroxidase ( p = 0.002), and glutathione ( p = 0.02) levels in ovarian tissues ( Figure 5 )

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However, it may be challenging to accurately control the dosage or adjust it to specific needs

To measure the intracellular level of GSH in yeast, YFP to CFP emission intensity ratio was recorded with 435 nm excitation filter and two emissions filters (485 nm for CFP and 535 nm for YFP) using LAS-AF software