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The pellet was resuspended in 500 L lysis buffer 2 (10 mmol/L Tris, pH 8.0, 200 mmol/L NaCl, 1 mmol/L EDTA, and 0.5 mmol/L EGTA with 0.1 mmol/L PMSF and the EDTA-free protease inhibitor cocktail) and incubated at room temperature for 10 minutes
Romero-Gomez M, Lawitz E, Shankar RR, Chaudhri E, Liu J, Lam RLH, et al
S2CID 919401
By this point, the cumulative additional fat loss attributable to AOD-9604 (beyond diet and exercise alone) is approximately 2-3 kg in responders
Warnings Do not exceed the recommended daily dose
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