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Collagen gel contraction assays Fibroblast cultures at passage 3 were cultured in six-well tissue culture plates (Gibco, Canada) in growth medium (DMEM, 10% FBS, penicillin, and streptavidin from Invitrogen, Canada)
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Epitope-tagged human FUS/TLS accumulated to a level equivalent to endogenous FUS/TLS in the parental cells, with a corresponding reduction in untagged endogenous FUS/TLS to about half its initial level, presumably through autoregulation (Fig
Many of these side effects can be reduced with the use of BPC-157
Genetically determined ancestry group based on ADMIXTURE with K = 10 (where K refers to the number of ancestral clusters inferred in the ADMIXTURE analysis), with grouping based on which 1000 Genomes phase 3 reference samples (with superpopulation labels as reference) showed the highest proportion for a given class
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