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These enzymes repress gene expression by removing acetyl groups from lysine residues on histones, leading to chromatin compaction

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Gene therapy This technique involves the direct transfer of therapeutic recombinant DNA into cells of the targeted site and involves both ex vivo and in vivo methods by either therapeutic gene transfer directly into the patient or using gene carriers such as vectors (viral or non-viral)

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Materials and methods Drugs and chemicals Powdered L-PAM and BSO (DL buthionine-( S,R )-sulfoximine) were purchased from Sigma-Aldrich (St Louis, MO, USA) and clinical grade BSO (L-buthionine ( S,R )-sulfoximine (50 mg/ml)) was provided by the National Cancer Institute (Bethesda, MD, USA)