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Non-cleavable linkers, commonly including maleimide and thiolactam, facilitate the conjugation of payload with antibodies or peptides by forming an amide bond (as in the linker in Kadcyla in Figure 6) or a thioether bond (as in the linker in Blenrep in Figure 6)

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The methylation at 75 CpG sites was unaltered by acute leptin treatment, suggesting that DNA methylation may not play a significant role in leptin-induced Bdnf gene expression