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Mosharov E, Cranford MR, Banerjee R: The quantitatively important relationship between homocysteine metabolism and glutathione synthesis by the transsulfuration pathway and its regulation by redox changes

2.3 Cell culture and treatment The human normal hepatic cell line LO2 (Shanghai Institute of Biological Sciences, Chinese Academy of Science, Shanghai, China) was maintained in DMEM(BI,United States) containing 10% FBS (Gibco, United States) and 1% penicillin/streptomycin (Gibco, United States) in a 5% CO2 humidified 37C incubator

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The supernatant (40 l) was added to a 160-l reaction mixture containing 143 mM of Na phosphate buffer (pH 7.5), 6.3 mM of EDTA, 0.22 mM of NADPH, and 20 l of 6 mM of 5,5-dithiobis-2-nitrobenzoic acid