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Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

In preclinical research, it is primarily applied to define how amylin receptor agonism alters feeding circuits, metabolic biomarkers, receptor trafficking, and peptide disposition across experimental models