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2012 GHK-Cu may prevent oxidative stress in skin by regulating copper and modifying expression of numerous antioxidant genes Cosmetics Study demonstrating GHK-Cu's regulation of antioxidant gene expression and copper-dependent protective mechanisms in skin tissue

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Quantitative real-time PCR (qPCR) was then carried out using SYBR Green PCR Master Mix on a real-time PCR system to assess gene expression levels