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doi: 10.1016/j.micpath.2021.104780, 53 KwasniewskiW.Wolun-CholewaM.KotarskiJ.WarcholW.KuzmaD.KwasniewskaA.et al

In sufficiently acidic environments, both carboxyl groups are protonated and the molecule becomes a cation with a single positive charge, HOOCCH(NH + 3 )(CH 2 ) 2 COOH

one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second

This assay operates on the principle that peroxides oxidize ferrous ions (Fe 2+ ) to ferric ions (Fe 3+ ) under acidic conditions