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Halo-tag was detected by quantitative Western blotting using anti-Halo primary antibody (Promega, #G9211, 1:1000) and a fluorescently labeled secondary antibody
The trials used different groups of participants and different study designs, so only a trial directly comparing tirzepatide and retatrutide could determine whether one consistently causes more side effects than the other
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10.1038/358209a0 12
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Jee et al., 2019), despite the fact it is recognized (Urist, 1996), and eventually jeopardizes the conclusions about these peptide activities and significance (Tarnawski and Ahluwalia, 2012
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