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Gel permeation chromatography (GPC) analysis (Prominence 501 system coupled with a Dawn-Heleos-, Wyatt Technology Europe GmbH, Dernbach, Germany) using GF-7MHQ (Showa Denko K.K., Tokyo, Japan) with 0.1 M carbonatebicarbonate buffer as the eluent revealed that the weight-average molecular weights (Mw) of the PEG and A45 were 2 10 and 7.6 10, respectively 29,30

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