Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
Key Features Research Use Only (RUO) compound 5 mg per bottle Spray format for convenient handling in laboratory settings Manufactured and packaged for research applications Product Specifications Compound Name: DSIP Form: Spray Total Quantity: 5 mg per bottle Grade: Research Use Only (RUO) Intended Use This product is intended solely for laboratory research and investigational purposes
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Schedule: Weekly subcutaneous injections for 1216 weeks (or longer as appropriate)
The ratio [GSH]/[GSSG] is thought to be around 100 for cells that are not under oxidative stress [41], and [GSH]/[GSSG] = 107.5 in our model cell