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The blood circulation of the nanoparticles was assessed upon intravenous injection into the tail vein of healthy female mice and determination of the metal content in the blood by ICP-MS

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Synthetic peptides are engineered to improve bioavailability, target selectivity, and half-life, often surpassing the pharmacokinetic limitations of natural peptides. Vlieghe et al., Drug Discovery Today Do Natural and Synthetic Peptides Have Different Clearance Times

The cells were fixed with 4% paraformaldehyde solution for 15 min, followed by washing with PBS buffer