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Oxidative DNA damage and antioxidant protein measurement in plasma by ELISA The concentration of free 8-hydroxy-2-deoxyguanosine (8-OHdG) in plasma samples was determined using a competitive ELISA kit provided by Stressmarq Biosciences, Canada (Catalog Number: SKC-120A), following the manufacturers protocol as previously described by us 30
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Gossypol is the main limiting factor in the application of cottonseed meal in grass carp feed production: involvement of growth, intestinal physical and immune barrier, and intestinal microbiota
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Benefits of medical supervision include baseline health assessment before starting protocols, appropriate bloodwork monitoring, professional guidance on dosing and duration, recognition of potential adverse effects, and integration with other health treatments
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