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Peroxynitrite (ONOO - ,0.1 M stock solution in 0.3 M NaOH-NaCl) was diluted to less than 1/100 with ice-cold water, just prior to quick exposure to HGF in PBS by vortexing at 1:2000 (see Figure 1a for the experimental design) 32

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Oxidative stress leads to imbalances in seminal plasma antioxidants, which is the main mechanism of a high semen viscosity, resulting in sperm DNA damage, sperm chromatin instability, membrane lipid peroxidation, and a low fertilization rate (Figure 1) (58)

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